For a successful grapevine breeding program, pollen viability, germination ability, and cultivar compatibility information are of vital importance. In this study, pollen viability, germination rates, pollen tube lengths, and pollen tube diameters of three grape cultivars [Kyoho (4n), Ekşi Kara (2n), and Gök Üzüm (2n)] were investigated. Pollen viability rates were determined using the IKI (iodine potassium iodide) staining test. In vitro, pollen germination rates were determined using a semi-solid medium containing 20% sucrose and 1% agar. As a result, significant differences were detected in pollen viability, germination rates, and pollen tube lengths of the examined grape cultivars. The first germination started at the 48th hour in all three grape cultivars. In cv. Ekşi Kara had the highest pollen viability while the lowest (3.05%) value in terms of pollen germination was determined. Pollen tube length and pollen tube diameter of the cv. Kyoho had higher values than the other two diploid cultivars. Pollen tube formation started in the first 48th hours, while pollen tube growth continued to increase until the 96th hours. As a result, it was confirmed that the flower structure of the cv. Ekşi Kara was functionally female. In suitable ecologies, diploid cv. Gök Üzüm and tetraploid cv. Kyoho were found to be usable as pollinators.
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Published on: Aug 17, 2024 Pages: 1-7
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DOI: 10.17352/gjfr.000025
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